A university freshman who has the opportunity to select the desired course of study believes that he/she has succeeded in choosing the right career path. However, even those students sometimes have serious anxiety about their careers in the labor markets. The reason why is that it is too difficult for them to predict their future careers due to rapid globalization and technology development and so on. In such circumstances, it is thought that the career management skills (hereinafter called “CMS”) development will play an important role for them in order to survive their uncertain life. Therefore, we investigated the difference of the following four CMS in the high and low career anxiety groups of freshmen. (1) Adopting to change in the society, (2) Managing goals and time, (3) Developing long-term career and learning and (4) Working effectively in diverse teams. Furthermore, we analyze which CMS are more effective in reducing career anxiety. As a result, the low-anxiety group scores were higher than high-anxiety group scores in all items. In particular, knowledge and skills of (1) and the knowledge of (3) are significantly different between two groups. This result indicates that acquiring these knowledge and skills in career guidance program is effective for reducing the career anxiety of university freshmen.